Proteasix Web-based Tool

Step 1: copy and paste your peptide list (tab-delimited text) and select the proteases you are interested in (TreeView on the right hand-side). Click here for examples: Example 1 (38 peptides); Example 2 (107 peptides). Please, do not exceed 750 peptides


Peptide list

Data from the User: Peptide list (information separated by a horizontal tab)

Peptide ID   Protein AC/Protein ID    Start AA   Stop AA

Cleavage site

This step obtains the cleavage site list using UniProtKB

Observed

This step checks if each cleavage site was previously observed

Predicted

This step uses a probabilistic model to check if cleavage site is cleaved by protease