Proteasix Web-based Tool

Step 1: copy and paste your peptide list (tab-delimited text) and select the proteases you are interested in (TreeView on the right hand-side). Click here for examples: Example 1 (38 peptides); Example 2 (107 peptides). Please, do not exceed 750 peptides

Peptide list

Data from the User: Peptide list (information separated by a horizontal tab)

Peptide ID   Protein AC/Protein ID    Start AA   Stop AA

Cleavage site

This step obtains the cleavage site list using UniProtKB


This step checks if each cleavage site was previously observed


This step uses a probabilistic model to check if cleavage site is cleaved by protease